Description
IMG-EK (pro-EMAPII), also known as p43, is a new cytokine that stimulates apoptosis
in endothelial cells. IMG-EK (pro-EMAPII) is one of the three auxiliary components
invariably associated with nine aminoacyl-tRNA synthetases as a
multienzyme complex . IMG-EK (pro-EMAPII), recombinant , is a 34.3kDa polypeptide
containing 312 amino acids, that induces cell death of endothelial
cells in vitro (1) and demonstrates potent anti-angiogenic activity
(2,3).
Formulation
IMG-EK (pro-EMAPII), recombinant, is supplied as a dried powder.
Optimal Biological Range
For most in vitro applications IMG-EK (pro-EMAPII) exerts its biological activity
in the concentration range 1nM~1uM.
Reconstitution
Dried IMG-EK (pro-EMAPII) is soluble in water and most aqueous buffers and can
be diluted into other buffered solutions and stored at -20¡ÆC for
future use.
Source
Recombinant DNA expressed in E. coli.
Storage Temperature
Store dried IMG-EK (pro-EMAPII), desiccated at -20¡ÆC. Avoid exposure to frequent
temperature changes. Store reconstituted IMG-EK (pro-EMAPII), in aliquots at
-20¡ÆC. The performance of this product is guaranteed for at least
six months from the date of purchase, if stored and handled properly.
Quality Control
Purity
>99% in SDS PAGE gel
Biological Activity
The ED50 for FGF, Basic, i.e., the concentration of factor that
produces one-half the maximal response, is determined in a cytotoxicity
bioassay using bovine aortal endothelial cells in DMEM with 10%
FBS and MTT assay. The ED50 value obtained is reported on the Product
Information Label affixed to this document.
References
A Cofactor of tRNA Synthetase, p43, Is Secreted to Up-regulate
Proinflammatory Genes. J. Biol. Chem., 276:23028-23033, 2001
A Novel Anti-tumor Cytokine Contains an RNA Binding Motif Present
in Aminoacyl-tRNA Synthetases. J. Biol. Chem., 275: 27062-27068,
2000
Dose-dependent Biphasic Activity of tRNA Synthetase-associating
factor, p43, in Angiogenesis. J. Biol. Chem., 277: 45243-45248,
2002
Copyright
(C) 2003 Imagene Co.,Ltd. All rights reserved.